Media

Ingredients

Reference

M1

Starch, 10 g; yeast extract, 4 g; peptone, 2 g; agar, 18 g and 1L of natural seawater

[3] [17] [25]

M6

Beef extract, 4 g; peptone, 4 g; yeast extract, 1; glucose, 10 g; NaCl, 20 g; agar, 20 g and 1L of distilled water

[10]

R2A

Yeast extract, 0.5 g; glucose, 0.5 g; peptone, 0.5 g; casein hydrolysate, 0.5 g; starch, 0.5 g; sodium pyruvate, 0.3 g; K₂HPO₄ 0.3 g; MgSO₄∙7H₂O, 0.05 g; agar, 18 g; 1L of seawater and distilled water (2:2) (Becton Dickinson)

[9]

Actinomycete isolation agar (AIA)

Sodium caseinate, 2 g; L-asparagine, 0.1 g; sodium propionate, 4 g; K₂HPO₄, 0.5 g; , MgSO₄∙7H₂O 0.2 g, ferrous sulphate 0.001 g, glycerol, 5 g; agar 15 g; 1L of seawater and distilled water (2:2) (Becton Dickinson)

[9]

SE Medium (sponge extract)

Sponge extract, 10 ml; agar, 15 g, 1L of seawater and distilled water (50%).

The water extract of sponge was prepared by grinding 20 g tissue in 200 ml sterile seawater and sterilized by filtration through a 0.2 μm pore size filter

[35]

AW Medium (agar water)

Agar, 15 g; 1L of seawater

[26] [40]

ISP4 (with 6% NaCl)

Starch, 10 g, K₂HPO₄, 1 g; MgSO₄∙7H₂O, 1 g; NaCl, 60 g; (NH₄)₂SO₄, 2 g; CaCO₃, 2 g; trace salt solution 1 ml; agar 20 g, 1L of distilled water.

[41]

SMP (sponge mannitol peptone)

Mannitol 500 mg, peptone 100 mg, 10% sponge extract and rifampicin (5 µg/ml) and 1L of seawater and distilled water (50%)

Modified form Jensen et al., 2005 [26]